evaluation of comprehensive 2d gas chromatography-time-of-flight mass spectrometry for 209 chlorinated biphenyl congeners in two chromatographic runs综合评价二维气体chromatography-time-of-flight质谱在两色谱运行209氯化联苯同系物.pdfVIP

SAGE-Hindawi Access to Research Chromatography Research International Volume 2011, Article ID 675920, 14 pages doi:10.4061/2011/675920 Research Article Evaluation of Comprehensive 2D Gas Chromatography-Time-of-Flight Mass Spectrometry for 209 Chlorinated Biphenyl Congeners in Two Chromatographic Runs L. I. Osemwengie and G. W. Sovocool National Exposure Research Laboratory Environmental Sciences Division, U.S. Environmental Protection Agency, P.O. Box 93478, Las Vegas, NV 89193-3478, USA Correspondence should be addressed to L. I. Osemwengie, osemwengie.lantis@ Received 29 July 2010; Accepted 28 February 2011 Academic Editor: Encarna Moyano Copyright © 2011 L. I. Osemwengie and G. W. Sovocool. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This research evaluates a recently developed comprehensive two-dimensional gas chromatography 2D GC coupled with a time-of-flight (TOF) mass spectrometer for the potential separation of 209 PCB congeners, using a sequence of 1D and 2D chromatographic modes. In two consecutive chromatographic runs, using a 40 m, Rtx-PCB column, and a 1 m DB-17 column, connected in series, 196 PCB congeners are distinguished, including 43 of the 46 pentachlorobiphenyl isomers. Some of the chlorinated biphenyls that could not be resolved chromatographically are resolved with the use of the “ortho effect,” which distinguishes PCB isomers having 2,2 -; and 2,2 6- chlorine substitution from those isomers without these substitutions. The result of this work falls short of our goal of se