高氧液对严重烧伤休克期羊淋巴循环TNF-α-IL-6-IL-8水平影响.docVIP

高氧液对严重烧伤休克期羊淋巴循环TNF-α\IL-6\IL-8水平影响[摘要]目的:研究高氧液对严重烫伤休克期羊淋巴循环TNF-α、IL-6、IL-8水平的影响。方法:将20只成年雌性羊随机分为2组,A高氧液组(10只)和B对照组(10只),2组均造成30%TBSAⅢ度烧伤,分别输入高氧液和平衡盐液, 经股前淋巴管插管收集淋巴液,用放射免疫分析法检测伤后6、24、48h 淋巴液TNF-α、IL-6、IL-8水平的变化,同时测定血氧饱和度(SaO2)。结果:烧伤休克期A组各时相点淋巴液TNF-α、IL-6、IL-8水平显著低于B组。A组SaO2明显高于B组。结论:高氧液对严重烧伤休克期羊淋巴循环有改善作用,降低淋巴液中TNF-α、IL-6、IL-8的水平。 [关键词]烧伤休克;高氧液;淋巴循环;TNF-α;IL-6;IL-8 [中图分类号]R644 [文献标识码]A [文章编号]1008-6455(2010)06-0856-03 The effects of hyperoxic fluid treatment on the of lymphatic TNF-α、IL-6、IL-8 levers of burn shock goats RAN Li,XIAO Hu,ZUO Ying-ying,WANG De-chang,WANG Yi-bing,HUO Ran,FENG Yong-qiang,LI Qiang (Department of Burn and Plastic Surger,Provincial Hospital Affiliated to Shandong University,Jinan 250021,Shandong,China) Abstract:ObjectiveTo study the effects of hyperoxic fluid treatment on the of lymphatic tumor necrosis factor (TNF-α),interleukin-6(IL-6)、interleukin-8(IL-8) levers of burn shock goats.Methods20 female adult goats were randomly divided into the hyperoxic fluid resuscitation group (group A,n=10),and the control (group B, n=10). After both groups were inflicted with 30% TBSA full-thickness skin burns,the concentrations of TNF-α,IL-6,IL-8 in lymph were measured at 6,24,48 hours post burn.ResultsThe levers of TNF-α,IL-6,IL-8 in lymph in group A were decreased than group B.ConclusionIn the burn shock stage,the hyperoxic fluid resuscitation could improve the lymph circulation and decrease the levers of TNF-α,IL-6,IL-8 in lymph. Key words:burn shock;hyperoxic fluid;lymph circulation;TNF-α;IL-6;IL-8 烧伤休克是烧伤后有效循环血量下降 ,引起组织和器官缺血、缺氧和细胞代谢紊乱的结果。烧伤早期休克的复苏好坏,直接关系到大面积烧伤救治的成功率 ,研究表明高氧液应用于烧伤休克治疗复苏能降低血液粘度和控制创面感染,增强烧伤创面的愈合,保护内脏功能[1-2]。目前国内外对烧伤休克期的研究主要局限于血循环,淋巴循环系统与烧伤休克关系的研究目前很少。 本实验主要研究烧伤休克期淋巴循环中炎性因子水平变化的变化及高氧液的影响,探讨淋巴循环系统发生的一系列病理生理的改变,从而拓宽对烧伤休克基础理论的认识。 1材料和方法 1.1 实验动物模型及处理:选用同一产地健康清洁级成年雌性波尔二代山羊(由山东省畜牧局羊场提供)20只,体重(45±5)kg。随机分为2组,A高氧液组(10只)和B对照组(10只),适应性喂养1周,实验前禁食24h,禁水8h,参照Demling法[4]建立股前淋巴瘘模型,收集胸部后侧、腹部、盆腔部及下肢等区域的淋巴液。将动物剃去两侧腹壁及右侧下肢3