罗非鱼尾胶原蛋白的提取与鉴定-食品科学.pdfVIP

※工艺技术 食品科学 2012, Vol. 33, No. 06 59 段宙位，申铉日 * ，陈秀明，王章晗 (海南大学食品学院，海南 海口 570228) 采用罗非鱼尾为原料，研究鱼尾脂溶性色素的去除方法及胶原蛋白的提取工艺，并测定胶原蛋白纯度， 鉴定胶原蛋白类型。结果表明，去除色素的最佳方案为混合溶剂氯仿 - 甲醇- 水(1:2:0.8，V/V) 、料液比 1:15(g/mL)、 时间36h，此条件下色素去除率达鱼尾湿质量的2.85% ；正交试验确定胶原蛋白提取的最佳工艺为盐酸胍浓度3mol/L、 胃蛋白酶添加量3.0%( 以粗蛋白量计) 、时间72h 、料液比 1:50(g/mL)、提取次数2 次，此条件下胶原蛋白提取率 达鱼尾湿质量的 4.96% ，占鱼尾粗蛋白含量的40.39% ；提取物中胶原蛋白含量达94.63% ，且显示Ⅰ型胶原蛋白 的特征。 胶原蛋白；色素；提取；鉴定 Extraction and Identification of Collagen from Tilapia Tail DUAN Zhou-wei ，SHEN Xuan-ri*，CHEN Xiu-ming ，WANG Zhang-han (College of Food Science, Hainan University, Haikou 570228, China) Abstract：The remove of lipid-soluble pigments and extraction of collagen from tilapia tails were studied. In addition, collagen purity and type were analyzed. The highest pigment removal rate, 2.85% (calculated on wet basis), was obtained after 36 h of treatment with a mixture of chloroform, methanol and water (1:2:0.8, V/V) at a material-to-liquid ratio of 1:1.5 (g/mL). The optimal conditions for collagen extraction, as determined by orthogonal array optimization, were guanidine hydrochloride concentration 3 mol/L, pepsin dose 3.0% (calculated based on crude protein weight), material-to-liquid ratio 1:50 (g/mL), and twice repeated extraction for 72 h each time. Under these conditions, the extraction efficiency of collagen was 4.96% (calculated on wet basis). In tilapia tails, collagen accounted for 40.39% of total crude protein. The extract obtained contained 94.63% of collagen and displayed the characteristics of collagen type Ⅰ. Key words ：collagen ；pigment ；extraction ；identification 中图分类号：TS254.9 文献标识码：A 文章编号：1002-6630(2012)06-0059-06 胶原蛋白是一