24 低乳糖奶水解单因素试验.doc

响应面法优化低乳糖奶水解工艺 徐晨.2，杜鉴3，陈历俊2，石维忱4 （1.大连工业大学，大连116034；2.北京三元食品股份有限公司,北京100085；3.中国食品添加剂和配料协会,北京100020；4.中国生物发酵产业协会，北京100037） 摘 要：牛奶营养丰富容易消化吸收人称“白色血液”，是最理想的天然食品。β-半乳糖苷酶对牛奶进行水解可生成易被人体吸收的葡萄糖、半乳糖及“双歧因子”低聚半乳糖，不仅能解决乳糖不耐症问题，还能增加牛奶的营养价值。本文在单因素初步试验的基础上，以低聚半乳糖合成率为响应指标，通过响应分析法对低乳糖水解工艺进行优化。结果表明，当反应温度为45.3℃、加酶量为2.5ml及反应时间为69.7min时，低聚半乳糖得率达到最大值6.15%。通过乳果糖试剂盒测定水解后低乳糖奶中乳果糖含量，平均含量为158.17mg/L。 关键词：低聚半乳糖；β-半乳糖苷酶；响应面法；低乳糖奶 Optimization of low-lactose milk manufacture using response surface methodology XU Chen1,2, CHEN Li-jun2, SHI Wei-chen3 ( 1. Dalian Polytechnic University, Dalian 116034; 2. Beijing Sanyuan Foods Co., Ltd, Beijing 100085; 3. China Fermentation Industry Association, Beijing 100037 ) Abstract：Nutrient-rich milk and dairy products, easy digestion and absorption, known as white blood , is the best natural food. China dairy industry has developed rapidly in recent years, but there is still a huge gap in average level compared to the world. And lactose intolerance is one of the most important reason for restricting the development of China dairy industry. Using β-galactosidase hydrolysis of milk can generate glucose, galactose and the bifidus factor galactooligosaccharide, which Not only solve the problem of lactose intolerance, but also increase the nutritional value of milk.On the basis of single factor experiment, the best hydrolysis technique parameter were acquired by response analysis in which the galactooligosaccharide synthesis rate was the main indicator. The results showed that when the reaction temperature was 45.3 ℃, enzyme concentration was 2.5ml and reaction time was 69.7min, the maximum galactooligosaccharide yield was 6.15%. The lactulose content in low-lactose milk was 158.17mg/L determined by lactulose kit.Key words: galactooligosaccharide; β-galactosidase; response surface methodology; low-lactose milk 0 引言 牛奶是迄今为止人们从自然界中所能够获得的最接近完美的一种食物，它不仅能够给人们提供身体及大脑生长发育所需要的优质的动物蛋白质、乳脂肪和维生素等，而且还是人体补钙的最佳选择[1]，但是乳糖不耐症限制了部分人群无法享用牛奶这一宝贵资源。利用β-半乳糖苷酶对牛奶进行水解可生成易被人体吸收的葡萄糖和半乳