马尾松组培生根研究 - 广西植物.doc

温度对马尾松组培单芽不定根发生的影响( 姚瑞玲, 王胤* （广西优良用材林资源培育重点实验室，广西林业科学研究院，南宁530002） 摘 要：马尾松（Pinus massoniana）组培生根困难，本研究通过探讨培养环境温度对马尾松组培单芽生根能力的影响，旨在为尾松组培苗生产提供参考。在严格控制光温条件的光照培养中，以马尾松无性组培继代芽为试验材料，研究不同温度处理下，马尾松组培单芽生根率、生根时间、根系条数、移栽成活率等生根能力指标及生根解剖构造的变化。数据采用SPSS 19.0统计分析软件，进行了单因素分析（ANOVA）的显著性检验及最小显著性差异法（LSD）的多重比较。在25℃最适温度培养下，马尾松组培生根效果较佳，移栽成活率最高可达98.1%。低温处理，生根时间显著延长，根系条数减少，生根率偏低；高温处理，根茎愈伤明显，移栽成活率显著降低。从生根解剖构造来看，马尾松不定根主要由维管形成层细胞分化而成。与25 ℃适温处理相比，20 ℃较低温下，细胞分裂活动缓慢，不定根原始体诱发较少；而在30 ℃较高温处理下，细胞活动旺盛，但髓射线与维管组织细胞染色颜色加深、排列紧密，将诱发的不定根原基细胞团区隔成带状或片状。 关键词：组培快繁生根率生根解剖不定根诱导 中图分类号：S722.8 文献标识码：A 文章编号：10.11931/guihaia.gxzw201604020 Effect of Temperature on Adventitious Root Formation of Tissue-cultured Shoots in Pinus massoniana YAO Rui-Ling, WANG Yin* (Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation，Guangxi Forestry Research Institute，Nanning 530002，China) Abstract: Pinus massoniana is one of the most important tree species for afforestation in southern China, and it is of great economic value and ecological benefit. P. massoniana is famous for the wide application of its timber and resin, being of very important high-value tree species. Breeding programs for P. massoniana were initiated in the 1980s in China. To capture the best genetic stock from the breeding program, an efficient system for rapid clonal propagation is of considerable value. Therefore, the application of biotechnology and especially tissue culture provides an important tool to propagate the selected genotypes. The regeneration of plants under aseptic and controlled environmental conditions is referred to as micropropagation because very small pieces of plant tissue or organs are used as starting vegetative tissue. Previous studies have revealed that P. massoniana was hard to root by tissue culture, and culture temperature was correlated to the stability of in vitro rooting in P. massoniana. This study was aimed to reveal the effect of temperature on in vitro rooting ability, and provide supports for the