A549细胞三维立体培养模型的建立及耐药机制.pdfVIP

.530. 中国老年学杂志2016 年2 月第 36 卷 A549 细胞三维立体培养模型的建立及耐药机制 车萍季旭明 吴智春王海瑞欧阳兵1 (山东中医药大学，山东济南 250355) 〔摘要〕 目的探讨肺腺癌A549 细胞三维立体(3D) 培养模型对顺铅(DDP) 的耐药性及其教附耐药机制。方法 胶原培养条件下建立 A549 三维立体培养模型，MTI 法检测平面(2D) 培养和3D 培养A549 细胞敏感性。采用整合素 ßl 激动剂(TSF) 和抑制剂(MAB) 干预，对其相关机 制进行研究。结果 A549 细胞3D 培养模型中可见细胞生长良好，细胞之间毅附形成典型的多细胞球。2D培养模型中A549 细胞对DDP 的 IC50 值 为 12.84μ， glmlβD 培养A549 细胞对 DDP 的 IC50 是3 1. 74 问/时，为 2D 培养的2.47 倍。与空白对照组比较，DDP 干预组及 MAB+DDP 组的抑制 率明显升高(PO.OI) ， TSF+DDP 组有较显著差别(PO. 05) ;与 DDP 干预组相比较，TSF+DDP 组细胞抑制率明显降低(PO. 05) ,MAB+DDP 组明显 升高(P0.05) 。结论 胶原法成功建立A549 细胞的3D 培养模型。与 2D 培养相比，该细胞模型具有耐药的特性，其机制可能与促进整合素 ßl 对 细胞的秸附作用有关。 〔关键词〕 三维立体培养;A549;DDP;整合素。1; 秸附耐药 〔中图分类号) R733. 矿2 (文献标识码) A (文章编号) 1005-9202(2016 )03~530~3;doi:l0. 3969/j. ÏS8n. l005-9202. 2016. 03.ω7 The establishment of three-dimensional cell culture model of A549 and the drug tolerance mechanism CHE Ping,JI Xu-Ming ,WU Zhi-Chun ,et al. Shandong University of Traditional Chin臼e Medicine ,Jinan 250355 ,Shando吨，China [Abstract) Objective To establish a three-dimensional culture model (3D) of human lung cancer A549 cells ,evaluate the tolerance of DDP to the 3D cells and study the cell adhesion mediated drug resistance( CAM-DR) mechanism. Methods 四e rat tail collagen solution was used to establish the 3 D cell culture system ,MTT method was used to detect the tolerance of 2D and 3 D culture A549 cells to DDP ,and discuss the CAM-DR mechanisrn. Results The irnproved method 3D culture systern of A549 cells was successfully established. The cells grew well and forrn sorne typical multicellular spheres. 四e IC50 value of DDP on 2D cells culture model was 12.84 f.LWml; the IC50 value of DDP on 3D cells culture model was 3 l. 74μglml ,2. 47 times of the 2D cells culture model. Comparing with that of blank control group