Cry1Ac青花菜对小菜蛾的抗性和产卵行为的影响.pdfVIP

中国蔬菜 2013 ( 16): 43-48 CHINA VEGETABLES CrylAc 青花菜对小菜峨的抗性和产卵行为 的影响 崔树松仪登霞杨丽梅*方智远刘玉梅庄木张扬勇孙培田 (中国农业科学院蔬菜花卉研究所，北京 100081 ) 摘 要:利用 3 个 CrylAc 蛋白表达量不同的青花菜株系，研究了其对抗性小菜娥和敏感小菜娥的抗 性和产卵选择行为的影响。实验室结果表明:在非 Bt 青花菜对照上，抗性小菜娥和敏感小菜峨的存活率 差异不显著;在 CrylAc 蛋白表达量为 167ng-il 的 Bt 青花菜上，敏感小菜峨的死亡率达到 100% ，而抗 性小菜峨的死亡率仅为 181)毛;在 CrylAc 蛋白表达量为224ng-dl 和 246ng-il 的 Bt 青花菜上，抗性小 菜娥和敏感小菜峨的死亡率均达到 100%。抗性小菜娥和敏感小菜峨在不同 CrylAc 蛋白表达量的 Bt 青花 菜上的产卵量没有显著差异，表明 CrylAc 蛋白表达量对小菜峨的产卵行为没有影响。温室试验结果与实 验室结果基本一致。 关键词: Bt 基因;青花菜;小菜峨;产卵行为;抗性治理 中圄分类号: S436.34 l.2+4 文献标识码:A 文章编号: 1000-6346 (2013) 16-0043-06 Effects of CrylAc Broccoli on Resistance to Diamondback Moth and Oviposition Behavior CUI Shu-song , YI Deng-xia , YANG Li-mei. , FANG Zhi-yuan , LIU Yu-mei , ZHUANG Mu , ZHANG Yang-yong , SUN Pei-tian (Institute ofVegetables and Flowers , Chinese Academy ofAgricultural Sciences , Beijing 100081, China) Abstract: In this study , effects of Bac il/us thuringiensis ( Bt) broccoli expressing different levels ofCrylAc protein on resistance to CrylAc-susceptible and CrylAc-resistant diamondback moth (DBM) and oviposition behavior were evaluated. The concentrations of CIγ1 Ac protein in these 3 Bt broccoli lines were 167 ng • g-I , 224 ng • g-I , and 246 ng • g-I fresh weight , respectively. Untransformed broccoli plants were used as control. The larval survival rate of diamondback moth on control leaves was not significantly different between resistant and susceptible DBM. The mortality of Cry1 Ac-resistant DBM was 18t?毛 on Bt plants expressing 167 ng • g-1 Cry 1Ac protein. The Cry 1Ac-susceptible larvae could not survive on all of the Bt broccoli. There was no significant difference in number of eggs laid by DBM adults among different Bt broccoli. The development of CrylAc-s1.Jsceptible and CrylAc-resistant DBM on Bt plants was also tested in greenho