Nogo-AB蛋白在RCS大鼠视网膜色素变性过程中的表达.docVIP

Nogo-A/B蛋白在RCS大鼠视网膜色素变性过程中的表达 耿慧萍，贺翔鸽 （400042重庆，第三军医大学大坪医院野战外科研究所眼科） [摘要] 目的 观察髓磷脂抑制蛋白NogoA/B在遗传性视网膜色素变性（retinitis pigmentosa, RP）遗传模型皇家学院外科大鼠（Royal college of surgeons rat, RCS）视网膜变性发展过程中的表达并探讨其意义。 方法 按出生后视网膜变性的发展程度将RCS-p+ (视网膜含色素的变性大鼠)分为P15d、P30d、P60d和P90d 4组，采用相应时期的RCS-rdy+p+(视网膜含色素的正常大鼠)作为对照，5只/时间点，利用免疫组织化学技术及免疫印记检测2组（共40只）RCS视网膜上NogoA/B蛋白的表达趋势。结果 与对照组相比，①P15d、P30d RCS-p+大鼠视网膜各层结构和细胞数目无明显变化，仅节细胞数目轻微减少；P60d、P90d大鼠视网膜外核层(outer nuclear layer，ONL)和内核层(inner nuclear layer，INL)结构出现明显紊乱，细胞数目的减少以ONL和神经节细胞层(ganglion cell layer，RGC)层为主。②IH显示NogoA/B蛋白在RCS-p+大鼠各时间段视网膜表达均为阳性，P15d开始出现，随变性过程呈升高趋势，主要表达在INL和RGC层，阳性细胞数目显著高于对照组。③WB免疫印记检测结果显示P15d、P30d、P60d和P90d NogoA蛋白表达量分别为0.827 37±0.212 92、1.110 19±0.089 99、1.315 52±0.028 57、1.268 81±0.080 42，与对照组相比有显著差异(P＜0.05)。NogoB2蛋白与Nogo-A蛋白表达趋势一致，但总体表达量较低。 结论Nogo-A蛋白可能参与了视网膜色素变性过程中视神经损伤后的再生修复抑制。 关键视网膜色素变性；Nogo蛋白；皇家外科学院大鼠RCSA Expression of Nogo-A/B proteins in the degeneration of RCS rat retinitis pigmentosa Geng Huiping，He Xiangge (Department of Ophthalmology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China)[Abstract] Objective To investigate the expression of Nogo-A/B proteins in the retina of Royal college of surgeons (RCS) rats (a classic genetic animal model with hereditary retinal degeneration) and its significance. Methods According to the development status of retinal degeneration, the RCS-p+ (RCS rats with inherited retinal dystrophy) rats were divided into 4 groups including a P15d group, a P30d group, a P60d group and a P90d group, and RCS-rdy+p+ (RCS rats without retinal degeneration) rats of the corresponding periods were used as control groups (C15d group, C30d group, C60d group and C90d group, n=5). Immunohistochemical method and Western blot were used to detect the expression patterns of Nogo-A/B proteins in the retinal tissues of the RCS rats (RCS-p+/RCS-rdy+p+). Results (1) Compared with the control groups, no obvious changes of the cell numbers and thickn