pcDNA3转染HPV16E6基因对人宫颈癌细胞C33A细胞生长及周期的影响.docVIP

pcDNA3转染HPV16E6基因对C33A细胞生长及周期的影响/M:17.0%)和C33A组(S28.0%;G2/M:16.6%)(P0.01)。空载体转染组和未转染组细胞周期的各期分布无明显差异(P0.05)。结论 HPV16E6可能通过某种途径参与了C33A细胞的周期调控，促进细胞分裂而促进C33A细胞的增殖。 关键词：人乳头瘤病毒HPV16E6 transfected by pcDNA3 influenced the growth and cycle of cervical carcinoma cell lines YANG Xiu-kun, CHEN Xuan-nan,JIANG Zheng （1. Jilin Province Tonghua City People’s Hospital OG, Jilin Tonghua, 134001;2. Jilin University Public Health Institute, Epidemiology and Statistics Department, Jilin Changchun, 130021; 3. Center for Disease Control and Prevention, Academy of Military Medical Sciences,Center for Infectious Disease Control, Bei jing 100071） (英文署名单位，请与中文对应) Abstract：Objective To investigate the effects of human papillomavirus types 16E6 on the proliferation，apoptosis of cervical carcinoma cell lines. Methods HPV16E6 gene was stably transfected into C33A cervical carcinoma cell line by liposome，and the expression of HPV16E6 mRNA and protein in transfected cell line were identified by RT-PCR and Western blotting analysis. The effects of growth，proliferation and cell cycles of cervical careinoma cell lines C33A by and flow cytometry analysis. Results C33A-E6 cell expressing pcDNA3-HPV16E6 plasmid and C33A-P expressing pcDNA3 plasmid were transfected stably by liposome. The growth of C33A-E6 was more rapid than C33A-P and C33A(P0.05). It indicated that HPV16E6 can promote the growth an proliferation of C33A. In C33A-E6 cell lines，the percent of stage DNA Pre-compound G1 boundary of the cell cycles and stage still G0/G1 (39.3%)were significantly less than control groups C33A-P(53.7%) and C33A(55.4%)(P0.01). But the percent of stages(38.8%) and the stage DNA after-compound G2 and the splitted stage M(G2/M:21.9%) were distinetly higher than control groups C33A-P(S:29.4;G2/M:17.0%) and C33A(s:28.0%; G2/M:16.58%) (P0.0l). However，there was significantly difference between C33A-P and C33A cell lines(P0.05). Conclusion HPV16E6 through some kind of way involved in cell cycle and promote cell divi