用于关节腔注射的马钱子碱壳聚糖温敏凝胶的研究Brucine-药学学报.PDF

652 药学学报 Acta Pharmaceutica Sinica 2012, 47 (5) : 652 656 用于关节腔注射的马钱子碱壳聚糖温敏凝胶的研究 1 2 2 1, 3* 陈志鹏 , 刘 文 , 陈洪轩 , 蔡宝昌 ( 1. 南京中医药大学药学院, 江苏 南京 210029, 2. 河南大学药学院, 河南 开封 475004, 3. 南京海昌中药集团, 江苏 南京 210061) 摘要: 利用壳聚糖-甘油-饱和硼砂溶液为主要材料构建一种用于关节腔注射、具有缓释作用的壳聚糖反相 温敏水凝胶系统, 并对其理化性质和生物相容性进行初步研究。以凝胶的形成和胶凝时间为指标, 考察壳聚糖浓 度、壳聚糖与甘油的比例、pH 值等因素对水凝胶理化性质的影响, 并对其体外释放行为、流变学、生物相容性 等特性进行表征。温敏凝胶在常温下为溶胶态, 当处于37 时转变为凝胶态, 并具有一定的缓释效果, 生物相容 性较高。 关键词: 马钱子碱; 关节腔注射; 反相温敏凝胶; 生物相容性 中图分类号: R943 文献标识码:A 文章编号: 05 13-4870 (2012) 05-0652-05 Brucine chitosan thermosensitive hydrogel for intra-articular injection 1 2 2 1, 3* CHEN Zhi-peng , LIU Wen , CHEN Hong-xuan , CAI Bao-chang (1. College of Pharmacy, Nanj ing University of Chinese Medicine, Nanj ing 210029, China; 2. College of Pharmacy, Henan University, Kaif eng 475004, China; 3. Nanj ing Haichang Group of Chinese Medicine, Nanj ing 210061, China) Abstract: The aim of this study was to develop a sustained release converse thermosensitive hydrogel for intra-articular inj ection using chitosan-glycerol-borax as matrix, its physical properties and biocompatibility were investigated. Taking gelation time and gelation condition as index, the influence of concentration of chitosan, ratio of chitosan to glycerol, pH on physical properties of hydrogel were investigated. And then the in vitro drug release, rheological properties and biocompatibility were studied. The thermosensitive hydrogel flows easily at room temperature and turns to gelation at bo