金针菇担孢子核相及遗传属性的研究.PDF

26 3 369~375 2007 Mycosystema ∗ 1 2 430070 273155 3 80.2%7.5%12.3% RAPD 10 4 4 RAPD Q939.5 A1672-6472200703-0369-0375 Studies on nuclear phase and genetic attribute of the basidiospores of Flammulina velutipes XU Zhi-Yong1 LIN Fan-Xue1,2 XIONG Jie1 MA Li1 LIN Fang-Can1* 1 2 ( The Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, China; Department of Biology, Jining College, Jining 273155, China) Abstract Nuclear phase and genetic attribute of the basidiospores were investigated for 3 strains of Flammulina velutipes. Fluorescence staining observation displayed that the majority of basidiospores were dikaryotic and the ratio of dikaryotic spores, monokaryotic ones and nuclear-free ones was 80.2%, 7.5% and 12.3% respectively. The mycelia derived from single spore isolates were confirmed as polykaryotic septate ones without clamp connection. In the mating test, mycelial protoplast pairs between single spore isolates of different strains formed colonies with clamp connection, while mycelial protoplast pairs between the same single spore isolates formed colonies without clamp connection, suggesting that the two nuclei of the spores possessed identical mating type factors. RAPD analysis indicated that the profiles of mycelial protoplasts obtained from same single spore isolates amplified