铁氧化细菌的分离及其浸出钼镍尾矿试验薛洪其1杨爱江12邓秋静.DOC

铁氧化细菌的分离及其浸出钼镍尾矿试验 薛洪其1，杨爱江1,2，邓秋静1,2，姚维3，黎丹丹1 (1.贵州大学 资源与环境工程学院贵阳 550025；2. 贵州大学 环境工程规划设计研究所，贵阳 550025；3.贵州江航环保科技有限公司，贵州遵义 563000) 摘要：从钼镍矿区矿坑废水中分离出1株嗜酸性硫杆菌，通过对该菌株形态及理化特征检测分析，初步鉴定为氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans，A.f菌)。以FeSO47H2O和钼镍尾矿作为能量来源对其驯化培养，并利用驯化与非驯化的A.f菌进行摇瓶浸出钼镍尾矿验。结果表明驯化后的A.f菌浸出率大小顺序NiZnAsMo，钼镍尾矿中Ni、Mo、Zn、As在驯化细菌作用下最大浸出率分别为62.41%、55.66%、41.87%12.04%，比非驯化组高2～8A.f菌矿过程中，Ni、ZnAs、Mo关键词：氧化亚铁硫杆菌；驯化；钼镍尾矿；浸出TF18 文献标志码：A 文章编号：1007-7545（2016）07-0000-00 Isolation of Iron Oxidization Bacterium and Bioleaching from Ni-Mo Tailings XUE Hong-qi1, YANG Ai-jiang1,2, DENG Qiu-jing1,2, YAO Wei3, LI Dan-dan1 (1. College of Resource and Environmental Engineering, Guizhou University, Guiyang 550025, China; 2. Institution of Environmental Engineering Planning and Design, Guizhou University, Guiyang 550025, China; 3. Guizhou Jianghang Environmental Protection Technology Company Limited, Zunyi 563000, Guizhou, China) Abstract: One acidophilic thiobacillus bacterium isolated from acid mine drainage of nickel-molybdenum was identified as Acidithiobacillus ferrooxidans (A.f) by morphological and physiological characteristics analysis. It was domesticated applying Ni-Mo ore tailings and FeSO4?7H2O as energy sources. Bioleaching of Ni-Mo ore tailings with domesticated and non-domesticated A.f was carried out in shake flasks. The results show that domesticated A.f was more conducive to leaching metals from tailings, the sequence of leaching rate of four target metals is NiZnAsMo with the maximum extraction rate of 62.41%, 55.66%, 41.87%, and 12.04%, which is 2~8 percent point higher than that of non-acclimated group. The bioleaching mechanisms were attributed to be oxidation by bacterial and chemical solubilization by microbially-produced H+ and Fe3+. H+ plays a dominant role in leaching of Ni and Zn from tailings, while leaching of As and Mo mainly relies on continued oxidation by Fe3+. Key words: Acidithiobacillus ferrooxidans; acclimatization; Ni-Mo tailings; bioleaching; mechani