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Hindawi Publishing Corporation Experimental Diabetes Research Volume 2012, Article ID 518426, 11 pages doi:10.1155/2012/518426 Research Article A PEDF-Derived Peptide Inhibits Retinal Neovascularization and Blocks Mobilization of Bone Marrow-Derived Endothelial Progenitor Cells Richard Longeras,1 Krysten Farjo,1 Michael Ihnat,2 and Jian-Xing Ma1 1 Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma, OK 73104, USA 2 Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma, OK 73104, USA Correspondence should be addressed to Jian-Xing Ma, jian-xing-ma@ouhsc.edu Received 1 April 2011; Accepted 27 April 2011 Academic Editor: Gian Paolo Fadini Copyright © 2012 Richard Longeras et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Proliferative diabetic retinopathy is characterized by pathological retinal neovascularization, mediated by both angiogenesis (involving mature endothelial cells) and vasculogenesis (involving bone marrow-derived circulating endothelial progenitor cells (EPCs)). Pigment epithelium-derived factor (PEDF) contains an N-terminal 34-amino acid peptide (PEDF-34) that has antiangiogenic properties. Herein, we present a novel finding that PEDF-34 also possesses antivasculogenic activity. In the oxygen- induced retinopathy (OIR) model using transgenic mice that have Tie2 promoter-driven GFP expression, we quantified Tie2GFP+ cells in bone marrow and peripheral blood by fluorescence-activated cell sorting (FACS). OIR significantly increased the number of circulating Tie2-GFP+ at P16, correlating with the