amelioration of glucose control mobilizes circulating pericyte progenitor cells in type 2 diabetic patients with microangiopathy改善血糖控制循环周皮细胞祖细胞动员的2型糖尿病患者微血管病.pdfVIP

Hindawi Publishing Corporation Experimental Diabetes Research Volume 2012, Article ID 274363, 8 pages doi:10.1155/2012/274363 Research Article Amelioration of Glucose Control Mobilizes Circulating Pericyte Progenitor Cells in Type 2 Diabetic Patients with Microangiopathy Gian Paolo Fadini,1, 2 Patrizia Mancuso,3 Francesco Bertolini,3 Saula de Kreutzenberg,1 and Angelo Avogaro1, 2 1 Department of Clinical and Experimental Medicine, University of Padova, 35128 Padova, Italy 2 Venetian Institute of Molecular Medicine, 35129 Padova, Italy 3 European Institute of Oncology, 20141 Milan, Italy Correspondence should be addressed to Gian Paolo Fadini, gianpaolofadini@ Received 5 December 2011; Accepted 20 December 2011 Academic Editor: Paolo Madeddu Copyright © 2012 Gian Paolo Fadini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Chronic diabetic complications result from an imbalance between vascular damage and regeneration. Several circulating lineage- committed progenitor cells have been implicated, but no data are available on pericyte progenitor cells (PPCs). Based on the evidence that PPCs increase in cancer patients after chemotherapy, we explored whether circulating PPC levels are affected by glucose control in type 2 diabetic patients, in relation to the presence of chronic complications. We enumerated peripheral blood PPCs as Syto16+CD45−CD31−CD140b+ events by flow cytometry at baseline and after 3 and 6 months of glucose control by means of add-on basal insulin therapy on top of oral agents in 38 poorly controlled type 2 diabetic patients. We found that, in patients with mi