an evaluation of mapia in michigan as an ante-mortem supplemental test for use in suspect tuberculosis cattle评估在密歇根mapia经补充测试用于怀疑结核病牛.pdfVIP

Hindawi Publishing Corporation Veterinary Medicine International Volume 2012, Article ID 674368, 6 pages doi:10.1155/2012/674368 Research Article An Evaluation of MAPIA in Michigan as an Ante-Mortem Supplemental Test for Use in Suspect Tuberculosis Cattle Scott D. Fitzgerald,1 Heather A. Grodi,1 and John B. Kaneene2 1 Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine and the Diagnostic Center for Population and Animal Health, Michigan State University, Lansing, MI 48910, USA 2 Center for Comparative Epidemiology, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA Correspondence should be addressed to Scott D. Fitzgerald, fitzgerald@ Received 19 December 2011; Accepted 13 February 2012 Academic Editor: Mitchell Palmer Copyright © 2012 Scott D. Fitzgerald et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The objective of this study was to make use of bovine tuberculosis suspect cattle from the state of Michigan to validate a multiantigen print immunoassay for use on sera to serve as an improved supplementary ante-mortem test to increase specificity of current tuberculosis testing methods. Over a 27-month period, 234 sera were collected and tested by MAPIA method, which was evaluated using four different interpretation criteria. These results were subsequently compared to final mycobacterial culture and PCR results obtained by the National Veterinary Services Laboratories, Ames, IA, which served as the true indicator of the cattle’s tuberculosis infection status. This study indicates that an interpretation criterio