analgesic effects and the mechanisms of anti-inflammation of hispolon in mice镇痛效果和hispolon在小鼠的抗炎机制.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 478246, 8 pages doi:10.1093/ecam/nep027 Original Article Analgesic Effects and the Mechanisms of Anti-Inflammation of Hispolon in Mice Heng-Yuan Chang,1 Ming-Jyh Sheu,2 Chun-Hung Yang,3 Tsung-Chun Lu,1 Yuan Shiun Chang,1 Wen-Huang Peng,1 Shyh-Shyun Huang,1 and Guan-Jhong Huang1 1 Institute of Chinese Pharmaceutical Sciences, Taichung 40402, Taiwan 2 School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan 3 Department of Pesticide Toxicology, Taiwan Agricultural Chemicals and Toxic substances Research institute, Wu Feng, Taichung 433, Taiwan Correspondence should be addressed to Guan-Jhong Huang, gjhuang@.tw Received 29 October 2008; Accepted 3 March 2009 Copyright © 2011 Heng-Yuan Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Hispolon, an active ingredient in the fungi Phellinus linteus was evaluated with analgesic and anti-inflammatory effects. Treatment of male ICR mice with hispolon (10 and 20 mg/kg) significantly inhibited the numbers of acetic acid-induced writhing response. Also, our result showed that hispolon (20 mg/kg) significantly inhibited the formalin-induced pain in the later phase (P .01). In the anti-inflammatory test, hispolon (20 mg/kg) decreased the paw edema at the fourth and fifth hour after λ-carrageenin (Carr) administration, and increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRx) in the liver tissue. We also demonstrated that hispolon significantly attenuated