analysis of protein from nasopharyngeal carcinoma cell line cne2 irradiated by x-ray using raman spectroscopy分析蛋白质的鼻咽癌细胞株cne2 x射线辐照下使用拉曼光谱.pdfVIP

Spectroscopy 27 (2012) 1–7 1 DOI 10.3233/SPE-2012-0569 IOS Press Analysis of protein from nasopharyngeal carcinoma cell line CNE2 irradiated by X-ray using Raman spectroscopy Lin Ou a,∗, Yang Chen b , Yangwen Huang c , Ying Su d , Duo Lin a , Changyan Zou d and Jinping Lei a a Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China b Zhicheng College, Fuzhou University, Fuzhou, China c Key Laboratory of Instrumentation Science Dynamic Measurement, Ministry of Education, North University of China, Taiyuan, China d Fujian Provincial Tumor Hospital, Fuzhou, China Abstract. Our work aims to explore Raman spectroscopy (RS) to study the effects of total protein of nasopharyngeal carcinoma (NPC) cell line CNE2 treated by different doses of X-ray irradiation. The total protein is extracted from the cell line after irradiation with no incubation (0 h) and with incubation for 72 h, respectively. Both paired-samples t test and support vector machine (SVM) are employed for statistical analysis of Raman spectrum. The results show that several Raman bands assigned to total protein can be detected; and the X-ray irradiation have various effects on different components in total protein, especially for the dose higher than 6 Gy, and also associated with the incubation time after irradiation. The differences of content between 0 h groups and 72 h groups appear at bands of C–S stretching, C–H stretching, O=C–O− stretching and L-phenylalanine. It indicated no significant diversification on structures of protein, while the content may be varied during irradiation. Our spectroscopic results reveal that RS analysis of total protein of cell line irradiated by X-ray, in conjunction with mathematical statistical model, can be a potential method to explore the Raman characteriza