analysis of the interactions of botanical extract combinations against the viability of prostate cancer cell lines分析交互作用的植物提取物组合对前列腺癌的可行性细胞系.pdfVIP

eCAM 2006;3(1)117–124 doi:10.1093/ecam/nel001 Original Article Analysis of the Interactions of Botanical Extract Combinations Against the Viability of Prostate Cancer Cell Lines Lynn S. Adams, Navindra P. Seeram, Mary L. Hardy, Catherine Carpenter and David Heber Center for Human Nutrition, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA Herbal medicines are often combinations of botanical extracts that are assumed to have additive or synergistic effects. The purpose of this investigation was to compare the effect of individual botanical extracts with combinations of extracts on prostate cell viability. We then modeled the interactions between botanical extracts in combination isobolographically. Scutellaria baicalensis, Rabdosia rubescens, Panax-pseudo ginseng, Dendranthema morifolium, Glycyrrhiza uralensis and Serenoa repens were collected, taxonomically identified and extracts prepared. Effects of the extracts on cell viability were quantitated in prostate cell lines using a luminescent ATP cell viability assay. Combina- tions of two botanical extracts of the four most active extracts were tested in the 22Rv1 cell line and their interactions assessed using isobolographic analysis. Each extract significantly inhibited the proliferation of prostate cell lines in a time- and dose-dependent