assessment of dna damage after photodynamic therapy using a metallophthalocyanine photosensitizerdna损伤的评估使用metallophthalocyanine光敏剂在光动力治疗.pdfVIP

Hindawi Publishing Corporation International Journal of Photoenergy Volume 2012, Article ID 281068, 10 pages doi:10.1155/2012/281068 Research Article Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer A. El-Hussein,1, 2 M. Harith,2 and H. Abrahamse1 1 Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South Africa 2 National Institute of Laser Enhanced Science, Cairo University, 12613 Giza, Egypt Correspondence should be addressed to H. Abrahamse, habrahamse@uj.ac.za Received 7 September 2011; Accepted 28 October 2011 Academic Editor: David Worrall Copyright © 2012 A. El-Hussein et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Photodynamic therapy (PDT) is a chemotherapeutic approach that utilizes a bifunctional reagent, a photosensitizer (PS) that local- izes to the target tissue relative to the surrounding tissue and is toxic when exposed to laser light. PDT rapidly induces cell death, inflammatory and immune reactions, and damage of the microvasculature. DNA damage results from a variety of factors including UV-light, X-rays, ionizing radiation, toxins, chemicals, or reactive oxygen species. The aim of this study was to determine the effect of PDT as well as the influence of presensitization leading to the adaptive response (AR) on the integrity of DNA. Lung (A549), breast (MCF-7), and esophageal (SNO) cancer cells and Zn sulfophthalocyanine as PS with irradiation conditions of 10 J/cm2 at 636 nm were used. Subcellular localization of PS, cell morphology, and viability after PDT and DNA da