assessment of reference values for copper and zinc in blood serum of first and second lactating dairy cows评估的参考价值血清铜和锌的第一和第二泌乳奶牛.pdfVIP

SAGE-Hindawi Access to Research Veterinary Medicine International Volume 2010, Article ID 194656, 8 pages doi:10.4061/2010/194656 Research Article Assessment of Reference Values for Copper and Zinc in Blood Serum of First and Second Lactating Dairy Cows 1 ¨ 2 1 ¨ 2 Markus Spolders, Martin Holtershinken, Ulrich Meyer, Jurgen Rehage, and Gerhard Flachowsky1 1 Institute of Animal Nutrition, Friedrich-Loeffl er-Institute (FLI)—Federal Research Institute for Animal Health, Bundesallee 50, 38116 Braunschweig, Germany 2 Clinic for Cattle, University of Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany Correspondence should be addressed to Markus Spolders, markus.spolders@fli.bund.de Received 8 October 2009; Revised 17 December 2009; Accepted 20 January 2010 Academic Editor: Kazim Sahin Copyright © 2010 Markus Spolders et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The influence of different copper and zinc contents in rations on blood serum concentrations in dependence on feeding (Groups A and B) and lactation (Lactation 1 and 2) was tested in a feeding trial with 60 German Holstein cows. All animals received a diet based on maize and grass silage ad libitum. 30 cows received a concentrate supplemented with copper and zinc as recommended (Group A), whereas the other 30 animals were offered a concentrate with roughly double the amount of copper and zinc (Group B). Blood samples were taken several times during the lactation to ana