assessment of the antioxidant and reactive oxygen species scavenging activity of methanolic extract of caesalpinia crista leaf评估的抗氧化和活性氧清除活动methanolic提取的石莲子冠叶.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 173768, 11 pages doi:10.1093/ecam/nep072 Original Article Assessment of the Antioxidant and Reactive Oxygen Species Scavenging Activity of Methanolic Extract of Caesalpinia crista Leaf Sourav Mandal, Bibhabasu Hazra, Rhitajit Sarkar, Santanu Biswas, and Nripendranath Mandal Division of Molecular Medicine, Bose Institute, P-1/12 CIT Scheme VIIM, Kolkata-700054, India Correspondence should be addressed to Nripendranath Mandal, mandaln@rediff Received 26 December 2008; Accepted 28 May 2009 Copyright © 2011 Sourav Mandal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. “Oxidative stress” is initiated by reactive oxygen species (ROS), which are responsible for majority of the diseases. However, antioxidants with ROS scavenging ability may have great relevance in the prevention of oxidative stress. The present study was undertaken, using a 70% methanolic extract of Caesalpinia crista leaves, to examine different in vitro tests in diversified fields including total antioxidant activity, scavenging activities for various ROS, iron chelating activity and phenolic and flavonoid contents. Total antioxidant activity was evaluated as trolox equivalent antioxidant capacity value of 0.546 ± 0.014. The extract was investigated for different ROS scavenging activities and IC50 values were found to be 0.44 ± 0.1 mg/ml, 24.9 ± 0.98 μg/ml, 33.72 ± 0.85 μg/ml, 61.13 ± 3.24 μg/mL and 170.51 ± 4.68 μg/mL for hydroxyl, superoxide, nitric oxide, singlet oxygen and hypochlorous acid, respectively; however, no