role of crotoxin, a phospholipase a2 isolated from crotalus durissus terrificus snake venom, on inflammatory and immune reactionscrotoxin角色,磷脂酶a2隔绝crotalus什麽terrificus蛇毒,炎症和免疫反应.pdf

Research Paper Mediators of Inflammation, 10, 125– 133 (2001) Background : Crotoxin (CTX) is a potent neurotoxin Role of crotoxin, a phospholipase from Crotalus durissus terrificus snake venom (CdtV) composed of two subunits: one without cata- A2 isolated from Crotalus durissus lytic activity (crotapotin), and a basic phospolipase terrificus snake venom, on A2 . Recent data have demonstrated that CdtV or CTX inhibit some immune and inflammatory reactions. inflammatory and immune Aim : The aim of this paper was to investigate the mechanisms involved in these impaired responses. reactions Materials and methods : Male Swiss mice were bled before and at different intervals of time after subcuta- neous injection of CTX or bovine serum albumin 1 1 Diva F. Cardoso , Monica Lopes-Ferreira , (BSA) (control animals). The effect of treatments on 2 2 circulating leukocyte mobilisation and on serum Eliana L. Faquim-Mauro , Mahasti S. Macedo and levels of interleukin (IL)-6, IL-10, interferon (IFN)-g Sandra H. P. Farsky3,C A and corticosterone were investigated. Spleen cells from treated animals were also stimulated in vitro with concanavalin A to evaluate the profile of IL-4, IL- 1Laboratory of Immunopathology and 3 Laboratory of 6, IL-10 or IFN-g secretion. Cytokine levels were Immunochemistry, Institute Butantan, Av. Vital determined by immunoenzymatic assay and corticos-