利用表达分析和基因沉默方法研究硫代硫酸硫转移酶基因-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2012, 38(2): 231239 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2012.00231 利用表达分析和基因沉默方法研究硫代硫酸硫转移酶基因TaTST 与小 麦抗白粉病反应的关系 贺 洋 岳洁瑜 王华忠* / , 300387 : , TaTST, 0~48 h 2 , , 2 TaTST , TaTST (virus-induced gene silencing, VIGS) TaTST , TaTST TaTST H2O2 , TaTST - : ; ; ; VIGS Gene Expression Profiling and Silencing Reveal the Relationship between TaTST, a Wheat Thiosulfate Sulfurtransferase Gene, and the Resistance Re- sponse of Wheat to Powdery Mildew * HE Yang, YUE Jie-Yu, and WANG Hua-Zhong School of Life Sciences / Tianjin Key Laboratory of Cyto-Genetical Molecular Regulation, Tianjin Normal University, Tianjin 300387, China Abstract: Plant thiosulfate sulfurtransferase (TST), which participates in sulfur metabolism, removal of cyanide, generation and removal of reactive oxygen species (ROS), is closely related to plant disease resistance. The wheat TST-encoding gene TaTST was induced by the powdery mildew pathogen fungus Blumeria graminis f. sp. tritici (Bgt) in both the resistant and the susceptible wheat near-isogenic lines. Two expression peaks of TaTST were found from 0 to 48 h after inoculation of Bgt, corresponding to the initial contact and recognition between the host cell