神经坏死病毒对卵形鲳鲹的致病性及外壳蛋白基因序列分析.pdfVIP

: 1674 - 5566( 2010) 04- 0482- 07 鲹的致病性及 1, 2 1 1 1 1 许海东 , 区又君 , 郭志勋 , 苏友禄, 王瑞旋 ( 1. , 5 10300; 2. , 20 1306) : 200 8 5, 鲹( Trachinotus ovatus) 幼鱼发生大规模死亡, 调查发现病 , , PCR ( NNV ) NNV , , , ( ECNNV ) 99. 2% , ( RGNNV ) , 4 , 100% , , 鲹大规模死 : 鲹; 神经坏死病毒; 序列分析 : S 94 1. 4 : A The pathogenicity of nervous necrosis virus toTrach inotus ovatus and sequence analysis of the coat protein gene 1, 2 1 1 1 1 XU H a idong , OU Y oujun , GUO Zh ixun , SU Y oulu , WANG Ruixuan ( 1. South China Sea F isheriesR esearch Institute, ChineseA cademy of F ishery S ciences, Guangzhou 510300, China; 2. Colleg e of F isheries and fie Science, Shanghai Ocean University, Shanghai 20 1306, China) Abstract: Inves iga ion on m ass m or ali y of Trachinotus ovatus juveniles cu l ured in sea cage in Zhan jiang w as carried ou . T he sym p om of he sick fish w as as fo llow s: he skin w as nigrescen , i sw am w is y or c ircularly, responded slow ly and anorex ia. T here w as no in fec ion of parasi es or bac eria. The fish nervou s necrosis v irus w as observed in he fish by PCR analysis. Two pairs of prmi ers w ere designed based on he hom ogenu s gene sequences from o her fish nervou s necrosis v iru s ( NNV ) subm i ed o G eneBank and he coa pro ein gene of Trachinotus ovatus nervou s necrosis viru s ( PNNV ) w as cloned and sequenced. A cco rd ing o he analysis o f smi ilari y and phy logenelysis, he coa pro e in gene from PNNV had he highes iden i y o f 99. 2% o Ep inephelus coioides nervou s necrosis v ir