肉果秤锤树核果中萌发抑制物质的初步研究.pdfVIP

肉果秤锤树核果中萌发抑制物质的初步研究 * 刘超，伏秦超，罗正敏，张西玉，胥成浩，唐梅 （乐山师范学院,林竹生态研究中心,四川乐山 614004） 摘 要： 以休眠期肉果秤锤树 (Sinojackia sarcocarpa L.Q .luo )核果的果肉、内果皮和种仁水浸提液和不同 浓度甲醇浸提液分别测定对白菜种子萌发和幼苗生长的影响，以研究肉果秤锤树核果中萌发抑制物质类型 和存在部位。结果：果肉随水提时间的延长，对白菜种子发芽和幼苗生长、根生长的抑制作用逐渐增强 （p 0.05 或p 0.01 ），而内果皮、种仁水浸提液的影响不明显（p0.05 ）；各部位甲醇浸提物均能显著抑 制白菜种子发芽和生长，且随浸提液质量分数的升高抑制效应越强；在相同浓度水平时，抑制效应果肉最 强（p0.01 ），种仁显著（p0.05 ）高于内果皮；果肉的甲醇浸提液抑制作用也显著高于其水浸提液。各处 理组白菜种子的根生长均比苗生长缓慢。表明：果肉、内果皮和种仁中均含酯溶性抑制物，主要存在于肉 果中，且果肉中还含一定量的水溶性抑制物。 关键词：肉果秤锤树；核果；水浸提液；甲醇浸提液；抑制物；发芽率 Preliminary Study on Germination Inhibitors from Sinojackia sarcocarpa Drupe LIU Chao, FU Qin-chao, LUO Zheng-min，ZHANG Xi-yu, XU Cheng-hao, TANG Mei （Research center of forest and bamboo bionomics, Leshan normal university, Leshan, sichuan, 614004 ） Abstract: In order to gain a deeper understanding on relevant dormancy mechanism and to explore effective methods for improving seed germination, the flesh, endocarp and the kernel (embryo +endosperm) of S.sarcocarpa seeds(drupe) were soaked in water for different time(48h, 96h, 144h) and different methanol solution (25%, 50%, 75%) to extract inhibitory substances, taking wild as materials, and effects of extracts on germination rate of cabbage seed were investigated, respectively. The results showed that there has a significant (p 0.05) or extremely significant (p 0.01) inhibiting effect on the germination, growth of the tall and the root under the treatment of flesh extract by water than the endocarp and the kernel water extracts(p 0.05), while there was a significant inhibiting effect on the germination rate of cabbage seeds and the growth of its height and root under the treatment of the flesh, endocarp and the kernel with the different methanol concentrations (25%, 50%, 75%) and the inhib