原创力文档a comparative study of the arabidopsis thaliana guard-cell transcriptome and its modulation by sucrose的比较研究拟南芥保卫细胞转录组由蔗糖及其调制.pdfVIP
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a comparative study of the arabidopsis thaliana guard-cell transcriptome and its modulation by sucrose的比较研究拟南芥保卫细胞转录组由蔗糖及其调制
A Comparative Study of the Arabidopsis thaliana Guard-
Cell Transcriptome and Its Modulation by Sucrose
1 2 2,3 1,4 1,5
George W. Bates *, David M. Rosenthal , Jindong Sun , Maitreyi Chattopadhyay , Emily Peffer ,
6 2 6
Jing Yang , Donald R. Ort , Alan M. Jones
1 Department of Biological Science, Florida State University, Tallahassee, Florida, United States of America, 2 Global Change and Photosynthesis Research Unit, Agricultural
Research Service, United States Department of Agriculture, Institute for Genomic Biology, University of Illinois, Urbana, Illinois, United States of America, 3 Pioneer Hi-Bred
International, Johnston, Iowa, United States of America, 4 Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland, United
States of America, 5 Department of Plant Sciences, University of California Davis, Davis, California, United States of America, 6 Departments of Biology and Pharmacology,
University of North Carolina, Chapel Hill, North Carolina, United States of America
Abstract
Microarray analysis was performed on RNA isolated from guard cells that were manually dissected from leaves of
Arabidopsis. By pooling our data with those of two earlier studies on Arabidopsis guard cell protoplasts, we provide a robust
view of the guard-cell transcriptome, which is rich in transcripts for transcription factors, signaling proteins, transporters,
and carbohydrate-modifying enzymes. To test the hypothesis that photosynthesis-derived sugar signals guard cells to
adjust stomatal opening, we determined the profile of genes expressed in guard cells from le
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