a differential effect of e. coli toxin-antitoxin systems on cell death in liquid media and biofilm formation大肠杆菌toxin-antitoxin系统的微分效应细胞死亡在液体媒体和生物膜的形成.pdfVIP

A Differential Effect of E. coli Toxin-Antitoxin Systems on Cell Death in Liquid Media and Biofilm Formation . . Ilana Kolodkin-Gal , Reut Verdiger , Ayalla Shlosberg-Fedida, Hanna Engelberg-Kulka* Department of Molecular Biology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel Abstract Toxin-antitoxin (TA) modules are gene pairs specifying for a toxin and its antitoxin and are found on the chromosomes of many bacteria including pathogens. Here we report how each of five such TA systems in E. coli affect bacterial cell death differently in liquid media and during biofilm formation. Of all these systems, only the TA system mazEF mediated cell death both in liquid media and during biofilm formation. At the other extreme, as our results have revealed here, the TA system dinJ-YafQ is unique in that it is involved only in the death process during biofilm formation. Cell death governed by mazEF and dinJ-YafQ seems to participate in biofilm formation through a novel mechanism. Citation: Kolodkin-Gal I, Verdiger R, Shlosberg-Fedida A, Engelberg-Kulka H (2009) A Differential Effect of E. coli Toxin-Antitoxin Systems on Cell Death in Liquid Media and Biofilm Formation. PLoS ONE 4(8): e6785. doi:10.1371/journal.pone.0006785 ´ ¨ Editor: Stefan Bereswill, Charite-Universitatsmedizin Berlin, Germany Received June 28, 2009; Accepted July 24, 2009; Published August 26, 2009 Copyright: 2009 Kolodkin-Gal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This research was supported by grant No. 177/07 from the Israel Science Foundation (ISF) administrated b