a model-based analysis of chemical and temporal patterns of cuticular hydrocarbons in male drosophila melanogaster基于模型的分析化学和表皮碳氢化合物在雄性果蝇的时间模式.pdfVIP

A Model-Based Analysis of Chemical and Temporal Patterns of Cuticular Hydrocarbons in Male Drosophila melanogaster ¤ Clement Kent, Reza Azanchi, Ben Smith , Adrienne Chu, Joel Levine* Department of Biology, University of Toronto at Mississauga, Mississauga, Ontario, Canada Drosophila Cuticular Hydrocarbons (CH) influence courtship behaviour, mating, aggregation, oviposition, and resistance to desiccation. We measured levels of 24 different CH compounds of individual male D. melanogaster hourly under a variety of environmental (LD/DD) conditions. Using a model-based analysis of CH variation, we developed an improved normalization method for CH data, and show that CH compounds have reproducible cyclic within-day temporal patterns of expression which differ between LD and DD conditions. Multivariate clustering of expression patterns identified 5 clusters of co-expressed compounds with common chemical characteristics. Turnover rate estimates suggest CH production may be a significant metabolic cost. Male cuticular hydrocarbon expression is a dynamic trait influenced by light and time of day; since abundant hydrocarbons affect male sexual behavior, males may present different pheromonal profiles at different times and under different conditions. Citation: Kent C, Azanchi R, Smith B, Chu A, Levine J (2007) A Model-Based Analysis of Chemical and Temporal Patterns of Cuticular Hydrocarbons in Male Drosophila melanogaster. PLoS ONE 2(9): e962. doi:10.1371/journal.pone.0000962 INTRODUCTION In order to study daily variation in these compounds we have Chemical communication is fundamentally important to the had to review methods for analyzing these compounds. We have biology of many organisms. For example, several reproductive characte