基于SiO2荧光纳米粒子快速检测果汁中大肠杆菌的免疫传感器研究.PDFVIP

8 5 Vol. 8 No. 5 2017 5 Journal of Food Safety and Quality May. , 2017 SiO 2 孙晓萌, 张志涛, 王轶鹏, 许 强, 孙雪梅, 刘文敏* (, 250062) 摘 要: 目的 SiO O :H 方法 2 157 7 SiO2 SiO2 , O157:H7 , -, O157:H7 结果 , O157:H7 (P=0.930); 15 min , 10 CFU/mL结论 O157:H7 , , 关键词: SiO2 ; O157:H7; ; ; Biosensor for rapid detection of Escherichia coli in juice by luminophore-doped silica * SUN Xiao-Meng, ZHANG Zhi-Tao, WANG Yi-Peng, XU Qiang, SUN Xue-Mei, LIU Wen-Min (Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China) ABSTRACT: Objective To design a new biosensor for rapid detection of Escherichia coli O157:H7 in juice by synthesized luminophore-doped silica (LDS) nanoparticles. Methods The fluorescent groups were embed into SiO2 nanoparticles to synthesize LDS by supramolecular combination method. Then E. coli O157:H7 specific monoclonal antibody was coupled to the surface of the dye doped nanoparticles. Finally, antibody-coated narnoparticles incorporated with the cells of O157:H7 by antig