亚精胺对淹水胁迫下玉米幼苗根系抗氧化代谢调控.docVIP

亚精胺对淹水胁迫下玉米幼苗根系抗氧化代谢调控 摘 要：为了探讨亚精胺对淹水胁迫下玉米幼苗根系的抗氧化代谢调控机制，本实验以ldquo;郑单958rdquo; （耐淹水能力弱）和ldquo;浚单22rdquo;（耐淹水能力强）为材料，研究了在淹水胁迫下，亚精胺对玉米幼苗根系中抗氧化代谢酶活性及代谢产物含量的影响。结果表明：在正常生长情况下，两个品种的玉米幼苗根系中抗氧化代谢酶活性及代谢产物含量变化不明显；经过淹水处理，玉米幼苗根部的过氧化氢酶（CAT）活性，超氧化物歧化酶（SOD）活性，抗坏血酸氧化酶（APX）活性，过氧化物酶（POD）活性均下降，超氧阴离子（O2-)产生速率加快，植株生长发育受到影响；而外源亚精胺（Spd）处理后，玉米幼苗CAT、SOD、APX、POD活性增加，O2-产生速率降低。这些结果表明，亚精胺能通过增加玉米幼苗根部抗氧化酶的活性缓解淹水胁迫对玉米幼苗的伤害，从而促进玉米幼苗的生长。关键词：亚精胺；玉米幼苗；抗氧化代谢；淹水胁迫7539 Regulation of Spermidine on Antioxidant Metabolism of Maize Seedling Roots under Waterlogging Stress Abstract: In order to explore the regulation mechanism of spermidine on antioxidant metabolism of maize seedling roots under waterlogging stress, taking Zhengdan 958 (weaker anti -flood) and Xundan 22 (stronger anti-flood) as materials, this paper was carried out to study the effects of exogenous spermidine(Spd) on the activity of antioxidant enzymes and the content of metabolites of maize seedlings under waterlogging stress. Results are as follows: the activities of catalase (CAT),superoxide dismutase(SOD), ascorbic acid oxidase (APX) , peroxidase (POD) and the content of metabolites of maize seedling roots had no obvious change under normal condition; under waterlogging stress, the activities of CAT, SOD, APX, and POD decreased and superoxide anion (O2-) generation rate increased . However, after treatment with spermidine, the activities of CAT, SOD, APX and POD increased and superoxide anion generation rate decreased in Maize seedling roots. The results showed that Spd could relieve the injury of waterlogging stress on maize seedlings via increasing the activity of antioxidase of maize seedling root, and promote the growth of maize seedlings. 亚精胺(Spd)在缓解逆境伤害的方面有着重要作用[6]。Spd不仅可以直接作为胁迫保护物质, 还可以作为胁迫信号传导中的信号分子，构建抗胁迫机制[6]。外源Spd还具有提高植物抵御干旱和高、低温伤害等的能力, 外施Spd可以提高玉米、小麦等植物的抗盐性和抗逆能力[7]。然而, 亚精胺对缓解作物涝逆境伤害的研究还鲜见报道。本试验通过在淹水胁迫下的玉米幼苗喷施Spd，探究Spd对淹水胁迫下玉米幼苗根系抗氧化代谢的影响，进而探索Spd调控淹水胁迫下玉米幼苗根系抗氧化代谢的机理。以期明确涝胁迫对玉米根系抗氧化活性的抑制效应和外源Spd对其伤害的调控能力, 为玉米的涝灾害缓解技术研发提