怀牛膝多糖提取及抗氧化活性.docVIP

怀牛膝多糖提取及抗氧化活性 摘要：本实验通过微波辅助提取和热水浸提两种方法，对怀牛膝多糖进行提取，并采用苯酚硫酸法测定多糖的含量，同时测定提取液的体外抗氧化活性。结果表明，微波辅助提取和热水浸提法对怀牛膝多糖提取率分别为74.81 mg/g和70.87 mg/g。怀牛膝多糖提取液具有明显的抗氧化活性，微波辅助提取液对超氧阴离子自由基和羟自由基的清除率分别为70.48%、45.54%。热水浸提液对超氧阴离子自由基和羟自由基的清除率分别为68.34%、40.44%。 关键词：怀牛膝；多糖；微波辅助提取；热水浸提；抗氧化活性 Study on the Extraction of Polysaccharide from Achyranthis Bidentatae and Its Antioxidant Activity Abstract: In this experiment, the polysaccharide of Achyranthes bidentata was extracted by microwave-assisted and hot-water extraction. The polysaccharide content was detected by phenol sulfuric acid method. At the same time, the an