小鼠 tnf-α elisa试剂盒说明书（Mouse tnf- alpha ELISA Kit）.docVIP

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小鼠 tnf-α elisa试剂盒说明书（Mouse tnf- alpha ELISA Kit）.doc

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小鼠 tnf-α elisa试剂盒说明书（Mouse tnf- alpha ELISA Kit）

小鼠 tnf-α elisa试剂盒说明书（Mouse tnf- alpha ELISA Kit） This kit is for research use only Test range: 31.2 pg/ml - 2000 pg/ml Minimum detection limit: 7.8 pg/ml Specificity: the kit can simultaneously detect natural or recombinant mouse TNF- alpha and has no cross reactivity with other related proteins. Validity: 6 months Prospective application: ELISA method for quantitative determination of TNF- alpha in serum, plasma, cell culture supernatant or other related biological fluids in mice Content. Explain 1. kit kept: -20 degrees (when not longer); 2-8 degrees (frequently used). 2., concentrated liquid wash at low temperature will be salted out, diluted in the water bath heating, help dissolve. 3., the English and Chinese manual may be out of order. Please use the English manual. 4. the newly opened enzyme plate hole may contain a small amount of water sample, which is a normal phenomenon and will not result in any test results What effect. Summary Tumor necrosis factor (tumor, necrosis, factor, TNF) is a cytokine with a variety of biological activity. Rabbit TNF- alpha The gene encodes a precursor protein, whose signal peptide is immobilized on the cell membrane and becomes an active transmembrane interferon (TNF) the molecular mass is 26 * 103, and the signal peptide is enzymatically removed to form secretory TNF- alpha, with a molecular mass of 17 * 103. TNF- alpha cells have a wide range of sources, including a variety of immune cells, endothelial cells, fibroblasts, epidermal cells, keratinocytes, Smooth muscle cells, astrocytes, osteoblasts and so on. TNF- alpha has a wide range of biological activities, such as: participation in inflammatory and immune response, anti-tumor, and participate in endotoxin resistance Such pathological process as causing cachexia and so on. It has dual functions. On the one hand, it regulates the body and regulates the physiological functions of the organism And anti infection and so on, on the other hand, if sustained release or excessiv