噬菌体phi29DNA包装马达磷脂膜嵌合体在单分子检测及纳米医学.PDF

第23卷第11期 生命科学 Vol. 23, No. 11 2011年11月 Chinese Bulletin of Life Sciences Nov., 2011 文章编号：1004-0374(2011)11-1114-16 噬菌体phi29 DNA包装马达磷脂膜嵌合体 在单分子检测及纳米医学领域的应用 耿　佳，郭培宣* (美国辛辛那提大学医学院/工程学院，纳米生物医学中心，辛辛那提 45267 ，美国) 摘　要：生命系统包含了具有不同功能的纳米机器和高度有序的大分子结构。所有的双链线性DNA 病毒 使用由ATP 驱动的纳米分子马达将其基因包装在蛋白质外壳内。噬菌体phi29 DNA 包装马达的核心组成部 分连接器已被成功嵌入到脂双层中，极为稳定且可用于离子和DNA 转运的精确测量。它在包装DNA 时具 有单向通行的阀门机制，同时其关闭和打开可由人工控制。这对于详细研究分子马达的操作机制及未来医 药应用中DNA 的包装、测序、采样和投递都具有重要意义。 关键词：纳米马达；连接器；离子通道；单通道电导；病毒组装；计量定量化；生物纳米技术；纳米生物 技术 中图分类号：Q735; TB383 文献标志码：A Membrane-embedded channel of bacteriophage phi29 DNA-packaging motor for single molecule sensing and nanomedicine GENG Jia, GUO Pei-Xuan* (Nanobiomedical Center, College of Engineering and College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA) Abstract: The genome of linear double-stranded DNA (dsDNA) viruses is packaged into a preformed procapsid fueled by ATP hydrolysis. The central channel component of the bacteriophage phi29 motor is a connector protein, which has been successfully inserted into a lipid bilayer and exhibited robust capability for extremely reliable and precise assessment of the transportation of ions and DNA by single channel studies. The motor channel exercised a one-way traffic property for dsDNA translocation with a valve mechanism in DNA-packaging. In addition, the opening and closure of the channel was also reversible and controllable. These findings have important implications since this artificial membrane-embedded channel would allow detailed investigations into discrete mech