连翘挥发油成分分析及抗病毒活性考察.docVIP

连翘挥发油成分分析及抗病毒活性考察[摘要] 目的：考察连翘挥发油及其主要成分α-蒎烯及β-蒎烯对甲、乙型流感病毒与乙肝病毒的活性。方法：①通过GC-MS分析翘挥发油的化学成分；②以2.2.15细胞为乙型肝炎病毒载体，分别测定它们抑制乙型肝炎病毒进行DNA复制和产生HBsAg、HBeAg的能力；③以MDCK（狗肾）细胞为病毒宿主，分别测定它们抑制病毒引起细胞病变的程度（CPE）。结果：①在最大无毒剂量时，即在连翘挥发油258.69 μg/ml、α-蒎烯258.69 μg/ml、β-蒎烯192.45 μg/ml时，均无抗乙肝病毒活性；②连翘挥发油α-蒎烯、β-蒎烯的浓度分别是192.5 μg/ml时，均无抗甲、乙型流感病毒活性；③连翘挥发油的主要成分是α-蒎烯及β-蒎烯，总相对含量为71.48%。结论：连翘挥发油、α-蒎烯及β-蒎烯均无抗甲、乙型流感病毒与乙肝病毒的活性。 [关键词] 连翘挥发油；α-蒎烯；β-蒎烯；流感病毒；乙肝病毒 [中图分类号] R284.1[文献标识码]A [文章编号]1673-7210（2011）03（b）-031-03 Component analysis of forsythia essential oil and study on their anti-viral activity XIAO Huimin, HE Yue, WANG Siwang, WANG Jianbo，XIE Yanhua, MIAO Shan Institute of Materia Medical, School of Pharmacology, the Fourth Military Medical University, Xi′an 710032, China [Abstract] Objective: To investigate the activity that inhibited influenza A and B virus and hepatovirus B (HBV) from Forsythia essential oil and its main components including α-pinene and β-pinene. Methods: ①Using GC-MS to analyse the components of Forsythia essential oil. ②Using 2.2.15 cells as HBV′s vector to determine the content of HBV DNA duplicated and HBsAg and HBeAg produced from them，respectively. ③Using MDCK cells as host cells to observe the cytopathic effect (CPE) resulting from their inhabiting influenza A and B virus. Results：①Under the maximum nontoxic doses, it was no inhibiting HBV′s activity that the concentration of Forsythia essential oil was 258.69 μg/ml and the concentration of α-pinene was 258.69 μg/ml and the concentration of β-pinene was 192.45 μg/ml. ②It was no inhibiting influenza A and B viruses′ activity that the concentration of Forsythia essential oil and α-pinene and β-pinene was 192.5 μg/ml, respectively. ③Forsythia essential oil′smain components were α-pinene and β-pinene the amount of which was 71.48%. Conclusion: Forsythia essential oil and α-pinene and β-pinene were no inhibiting influenza A and B viruses and HBV′s activity. [Key words] Forsythia essential oil; α-pinene; β-pin