大鼠坐骨神经移植神经鞘瘤模型的建立.pdfVIP

中华神经外科杂志2016 年 11 月第32 卷第 11 期 Chin J Neurosu眩， November 2016 , Vol. 32 , No. 11 . 1151 . ·基础论著· 大鼠坐骨神经移植神经黯瘤模型的建立 李仕维刘主楠周强意张晶张j愤杨智君王博赵赋 [摘要] 目的 建立大鼠坐骨神经移植神经销瘤模型，为临床治疗神经鞠瘤的研究提供稳定、 有效的动物模型。方法 健康成年雄性 SD 大鼠 15 只，采用随机数字法随机均分为原代模型组、RT4 模型组及对照组，每组5 只。分别给予3 组大鼠坐骨神经鞠内注射等量的人前庭神经鞠瘤原代细胞、 大鼠神经喃瘤 RT4 细胞悬液及生理盐水。术后 1 周起对各组大鼠的坐骨神经功能进行测试。术后 4 周观察坐骨神经的成瘤情况，并行病理学检查确定肿瘤的组织学类型。结果 2 个模型组与对照组 比较，术后术侧后肢五趾间距均明显变小，术后第 14 天开始差异有统计学意义(P 0. 05); 与健侧比 较，2 个模型组术侧后肢五趾间距变小，术后第7 天开始差异有统计学意义(P O. 05); 与对照组比 较，2 个模型组大鼠在支架上停留时间均缩短，术后第 7 天开始差异即有统计学意义(PO.OI) 。 2 个模型组大鼠注射肿瘤细胞4 周内均在坐骨神经成瘤，肿瘤标本经 HE 染色后确认为神经销瘤，免 疫组化染色显示 S-1∞在细胞膜和细胞质均呈阳性表达。结论 大鼠坐骨神经移植神经鞠瘤细胞后 可在移植部位成瘤。 [关键词] 神经鞠瘤; 模型，动物; 肿瘤移植; 坐骨神经; 细胞系，肿瘤 基金项目:北京市卫生系统高层次卫生技术人才培养计划(2014-2-008) ;国家自然科学基金 ;北京市自然科学基金(7162057) The establishment of schwannoma xenograftmodel within the scia创c nerve of rats Li Shiwei , Liu Pina.n , Zhou Qiangyi , Zhang Jing , Zhαng Shun , Yang Zhijun , Wα， ng Bo , Zhao Fu. 拿 Department of Neurosurgery , Beijing Tiαntan Hospital , Capital Medical University , Beijing 100050 , Chinα Corresponding author: Zhao Fu , Email: z归ofu1024@126. com [Abstract 1 Objective To establish a stable and effective schwannoma xenograft model in ra臼 for the study of its clinical treatment. Methods Fifteen rats were randomly divided into 3 groups according to random number table. RT4 cells and human schwannoma cells were injected into the sheath of sciatic nerve in the model groups , and normal saline was injected into the control group. Function tests of sciatic nerves were performed since the first week after tumor implantation. We observed the tumors during the following 4 weeks ,which were identified by pathological examination. Results Compared with controls , the space b