association between the perioperative antioxidative ability of platelets and early post-transplant function of kidney allografts a pilot study围手术期抗氧化能力之间的联系和移植后早期血小板功能的肾移植一个试点研究.pdfVIP

Association between the Perioperative Antioxidative Ability of Platelets and Early Post-Transplant Function of Kidney Allografts: A Pilot Study 1. 1 . ´ 2 Barbara Dołe˛ gowska , Wojciech Błogowski * , Leszek Domanski 1 Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, Szczecin, Poland, 2 Department of Nephrology, Transplantation and Internal Medicine, Pomeranian Medical University, Szczecin, Poland Abstract Background: Recent studies have demonstrated that the actions of platelets may unfavorably influence post-transplant function of organ allografts. In this study, the association between post-transplant graft function and the perioperative activity of platelet antioxidants was examined among kidney recipients divided into early (EGF), slow (SGF), and delayed graft function (DGF) groups. Methodology/Principal Findings: Activities of superoxide dismutase, catalase, glutathione transferase (GST), glutathione peroxidase, and glucose-6-phosphate dehydrogenase (G6P) were determined and levels of glutathione, oxidized glutathione, and isoprostane were measured in blood samples collected immediately before and during the first and fifth minutes of renal allograft reperfusion. Our results demonstrated a significant increase in isoprostane levels in all groups. Interestingly, in DGF patients, significantly lower levels of perioperative activity of catalase (p,0.02) and GST (p,0.02) were observed. Moreover, in our study, the activity of platelet antioxidants was associated with intensity of perioperative oxidative st