associations of fatty acids in cerebrospinal fluid with peripheral glucose concentrations and energy metabolism协会的脂肪酸与外围脑脊液能量代谢和血糖浓度.pdfVIP

Associations of Fatty Acids in Cerebrospinal Fluid with Peripheral Glucose Concentrations and Energy Metabolism 1,2 3 2,4 2 3,5 Reiner Jumpertz *, Ana Guijarro , Richard E Pratley , Clinton C. Mason , Daniele Piomelli , Jonathan Krakoff2 ¨ ¨ ´ ¨ 1 Klinik fur Endokrinologie, Diabetes und Ernahrungsmedizin, Charite Universitatsmedizin, Berlin, Germany, 2 Phoenix Epidemiology and Clinical Research Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Phoenix, Arizona, United States of America, 3 Department of Pharmacology, University of California Irvine, Irvine, California, United States of America, 4 Sanford Burnham Medical Research Institute, Orlando, Florida, United States of America, 5 Drug Discovery and Development, Italian Institute of Technology, Genoa, Italy Abstract Rodent experiments have emphasized a role of central fatty acid (FA) species, such as oleic acid, in regulating peripheral glucose and energy metabolism. Thus, we hypothesized that central FAs are related to peripheral glucose regulation and energy expenditure in humans. To test this we measured FA species profiles in cerebrospinal fluid (CSF) and plasma of 32 individuals who stayed in our clinical inpatient unit for 6 days. Body composition was measured by dual energy X-ray absorptiometry and glucose regulation by an oral glucose test (OGTT) followed by measurements of 24 hour (24EE) and sleep energy expenditure (SLEEP) as well as respiratory quotient (RQ) in a respiratory chamber. CSF was obtained via lumbar punctures; FA concentrations were measured by liquid chromatography/mass spectrometry. A