asymmetry of chromosome replichores renders the dna translocase activity of ftsk essential for cell division and cell shape maintenance in escherichia coli不对称的染色体replichores呈现ftsk的dna移位酶活动必不可少的细胞分裂和细胞形状维护在大肠杆菌.pdfVIP

Asymmetry of Chromosome Replichores Renders the DNA Translocase Activity of FtsK Essential for Cell Division and Cell Shape Maintenance in Escherichia coli 1,2,3 1,2 3 4 1,2 Christian Lesterlin *, Carine Pages , Nelly Dubarry , Santanu Dasgupta , Franc¸ois Cornet * ´ ´ ´ ´ ´ 1 Laboratoire de Microbiologie et de Genetique Moleculaire, Centre National de la Recherche Scientifique, Toulouse, France, 2 Universite de Toulouse, Universite Paul ´ ´ ´ Sabatier, Toulouse, France, 3 Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France, 4 Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden Abstract Bacterial chromosomes are organised as two replichores of opposite polarity that coincide with the replication arms from the ori to the ter region. Here, we investigated the effects of asymmetry in replichore organisation in Escherichia coli. We show that large chromosome inversions from the terminal junction of the replichores disturb the ongoing post-replicative events, resulting in inhibition of both cell division and cell elongation. This is accompanied by alterations of the segregation pattern of loci located at the inversion endpoints, particularly of the new replichore junction. None of these defects is suppressed by restoration of termination of replication opposite oriC, indicating that they are more likely due to the asymmetry of replichore polarity than to asymmetric replication. Strik