barium promotes anchorage-independent growth and invasion of human hacat keratinocytes via activation of c-src kinase促进钡anchorage-independent增长和入侵人类hacat角质细胞通过c - src激酶的活化.pdfVIP

Barium Promotes Anchorage-Independent Growth and Invasion of Human HaCaT Keratinocytes via Activation of c-SRC Kinase 1,2 1 1 3 2 Nguyen Dinh Thang , Ichiro Yajima , Mayuko Y. Kumasaka , Shoko Ohnuma , Takeshi Yanagishita , 4 1 2 1,3 Rumiko Hayashi , Hossain U. Shekhar , Daisuke Watanabe , Masashi Kato * 1 Units of Environmental Health Sciences, Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai-shi, Aichi, Japan, 2 Department of Dermatology, School of Medicine, Aichi Medical University, Nagakute-cho, Aichi, Japan, 3 Voluntary Body for International Health Care in Chubu University, Kasugai-shi, Aichi, Japan, 4 Aichi Prefectural Institute of Public Health, Nagoya-shi, Aichi, Japan Abstract Explosive increases in skin cancers have been reported in more than 36 million patients with arsenicosis caused by drinking arsenic-polluted well water. This study and previous studies showed high levels of barium as well as arsenic in the well water. However, there have been no reports showing a correlation between barium and cancer. In this study, we examined whether barium (BaCl2) may independently have cancer-related effects on human precancerous keratinocytes (HaCaT). Barium (5–50 mM) biologically promoted anchorage-independent growth and invasion of HaCaT cells in vitro. Barium (5 mM) biochemically enhanced activities of c-SRC, FAK, ERK and MT1-MMP molecules, which regulate anchorage-independent growth and/or invasion. A SRC kinase specific inhibitor, protein phosphatase 2 (PP2), blocked ba