barley hvhma1 is a heavy metal pump involved in mobilizing organellar zn and cu and plays a role in metal loading into grains大麦hvhma1重金属泵参与动员organellar锌和铜金属加载到谷物中扮演了重要的角色.pdfVIP

Barley HvHMA1 Is a Heavy Metal Pump Involved in Mobilizing Organellar Zn and Cu and Plays a Role in Metal Loading into Grains 1,2 2 2 3 4 3 Maria Dalgaard Mikkelsen , Pai Pedas , Michaela Schiller , Eva Vincze , Rebecca F. Mills , Søren Borg , 1,2 2 4 1,2 3 Annette Møller , Jan K. Schjoerring , Lorraine E. Williams , Lone Baekgaard , Preben Bach Holm , Michael G. Palmgren1,2* 1 Centre for Membrane Pumps in Cells and Disease (PUMPKIN), Danish National Research Foundation, Frederiksberg, Denmark, 2 Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark, 3 Department of Molecular Biology and Genetics, Research Centre Flakkebjerg, Aarhus University, Slagelse, Denmark, 4 Centre for Biological Sciences, University of Southampton, Southampton, Hampshire, United Kingdom Abstract Heavy metal transporters belonging to the P1B-ATPase subfamily of P-type ATPases are key players in cellular heavy metal homeostasis. Heavy metal transporters belonging to the P1B-ATPase subfamily of P-type ATPases are key players in cellular heavy metal homeostasis. In this study we investigated the properties of HvHMA1, which is a barley orthologue of Arabidopsis thaliana AtHMA1 localized to the chloroplast envelope. HvHMA1 was localized to the periphery of chloroplast of leaves and in intracellular compartments of grain aleurone cells. HvHMA1 expression was significantly higher in grains compared to leaves. In leaves, HvHMA1 expression was moderately induced by Zn deficiency, but reduced by toxic levels of Zn, Cu and