beneficial effects of sitostanol on the attenuated immune function in asthma patients results of an in vitro approach有利影响减毒的sitostanol哮喘患者免疫功能的体外方法的结果.pdfVIP

Beneficial Effects of Sitostanol on the Attenuated Immune Function in Asthma Patients: Results of an In Vitro Approach ¨ 1 1 1 2 ¨ 2 Florence Brull , Ronald P. Mensink , Mandy F. Steinbusch , Constanze Husche , Dieter Lutjohann , 3 1 Geert-Jan Wesseling , Jogchum Plat * 1 Department of Human Biology, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands, 2 Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, Bonn, Germany, 3 Department of Respiratory Medicine, Maastricht University Medical Centre+, Maastricht, The Netherlands Abstract Background: In vitro and animal studies have suggested that plant sterols and stanols increase cytokine production by T- helper-1 cells. This may be beneficial for patient groups characterized by a T-helper-2 dominant immune response, e.g. asthma patients. (1) to evaluate whether sitostanol induces a T-helper-1 shift in peripheral blood mononuclear cells (PBMCs) from asthma patients, and (2) to unravel the role of regulatory T-cells in this respect. Methodology/Principal Findings: PBMCs from 10 asthma patients and 10 healthy subjects were isolated and incubated with 1.2 mM sitostanol, while stimulated with 5 mg/ml PHA. Similar amounts of cholesterol were used to determine whether effects were specific for plant stanols or for sterols in general. Changes in cytokine production were measured using antibody arrays and ELISAs. Changes in regulatory T-cell population size were measured by flow cytometry, using intracellular Foxp3 staining. Sitostanol increased production