asymmetric divergence in structure and function of hcn channel duplicates in ciona intestinalis不对称的分歧在hcn通道的结构和功能重复ciona intestinalis.pdfVIP

Asymmetric Divergence in Structure and Function of HCN Channel Duplicates in Ciona intestinalis 1 1 1 2 1 Heather A. Jackson , Andrew Hegle , Hamed Nazzari , Timothy Jegla , Eric A. Accili * 1 Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada, 2 Department of Biology, Eberly College of Science, Pennsylvania State University, University Park, Pennsylvania, United States of America Abstract Hyperpolarization-activated Cyclic Nucleotide (HCN) channels are voltage-gated cation channels and are critical for regulation of membrane potential in electrically active cells. To understand the evolution of these channels at the molecular level, we cloned and examined two of three HCN homologs of the urochordate Ciona intestinalis (ciHCNa and ciHCNb). ciHCNa is like mammalian HCNs in that it possesses similar electrical function and undergoes N-glycosylation of a sequon near the pore. ciHCNb lacks the pore-associated N-glycosylation sequon and is predictably not N-glycosylated, and it also has an unusual gating phenotype in which the channel’s voltage-sensitive gate appears to close incompletely. Together with previous findings, the data support an evolutionary trajectory in which an HCN ancestor underwent lineage-specific duplication in Ciona, to yield one HCN with most features that are conserved with the mammalian HCNs and another HCN that has been uniquely altered. Citation: Jackson HA, Hegle A, Nazzari H, Jegla T, Accili EA (2012) Asymmetric Divergence in Structure and Function of HCN Channel Duplicates in Ciona intestinalis. PLoS ONE 7(11): e47590. doi:10.1371/journal.pone.0047590 Editor: Zhe Zhang, Virginia