astrocyte-derived tissue transglutaminase interacts with fibronectin a role in astrocyte adhesion and migrationastrocyte-derived组织转谷氨酰胺酶与纤连蛋白交互作用在星形胶质细胞粘附和迁移.pdfVIP

Astrocyte-Derived Tissue Transglutaminase Interacts with Fibronectin: A Role in Astrocyte Adhesion and Migration? 1¤a ´ 1 1¤b 2 1 Miriam E. van Strien , John J. P. Breve , Silvina Fratantoni , Marco W. J. Schreurs , John G. J. M. Bol , 1 1 1 Cornelis A. M. Jongenelen , Benjamin Drukarch , Anne-Marie van Dam * 1 Department of Anatomy and Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, Amsterdam, The Netherlands, 2 Department of Pathology, VU University Medical Center, Neuroscience Campus Amsterdam, Amsterdam, The Netherlands Abstract An important neuropathological feature of neuroinflammatory processes that occur during e.g. Multiple Sclerosis (MS) is the formation of an astroglial scar. Astroglial scar formation is facilitated by the interaction between astrocytes and extracellular matrix proteins (ECM) such as fibronectin. Since there is evidence indicating that glial scars strongly inhibit both axon growth and (re)myelination in brain lesions, it is important to understand the factors that contribute to the interaction between astrocytes and ECM proteins. Tissue Transglutaminase (TG2) is a multifunctional enzyme with an ubiquitous tissue distribution, being clearly present within the brain. It has been shown that inflammatory cytokines can enhance TG2 activity. In addition, TG2 can mediate cell adhesion and migration and it binds fibronectin with high affinity. We therefore hypothesized that TG2 is involved in astrocyte-fibronectin interactions. Our studies using primary rat astrocytes show that