beneficial effects of estrogen in a mouse model of cerebrovascular insufficiency有利影响雌激素的脑血管机能不全的小鼠模型.pdfVIP

Beneficial Effects of Estrogen in a Mouse Model of Cerebrovascular Insufficiency 1. 1. 1 1 2 Naohito Kitamura , Runa Araya , Moeko Kudoh , Haruo Kishida , Tetsuya Kimura , Miyuki 2 2 3 3 4 4 Murayama , Akihiko Takashima , Yuriko Sakamaki , Tsutomu Hashikawa , Shingo Ito , Sumio Ohtsuki , 4 ¨ 5 1 Tetsuya Terasaki , Jurgen Wess , Masahisa Yamada * 1 Yamada Research Unit, RIKEN Brain Science Institute, Saitama, Japan, 2 Laboratory for Alzheimer’s Diseases, RIKEN Brain Science Institute, Saitama, Japan, 3 Research Resource Center, RIKEN Brain Science Institute, Saitama, Japan, 4 Department of Molecular Biopharmacy and Genetics, Tohoku University, Sendai, Japan, 5 Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland, United States of America Abstract Background: The M5 muscarinic acetylcholine receptor is known to play a crucial role in mediating acetylcholine dependent dilation of cerebral blood vessels. Previously, we reported that male M5 muscarinic acetylcholine knockout mice (M5R2/ 2 mice) suffer from a constitutive constriction of cerebral arteries, reduced cerebral blood flow, dendritic atrophy, and short- term memory loss, without necrosis and/or inflammation in the brain. Methodology/Principal Findings: We employed the Magnetic Resonance Angiography to study the area of the basilar artery in male and female M5R 2/ 2 mice. Here we show that female M5R2/ 2 mice did not show the reduction in vascular area observed in male M5R2/