原创力文档- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
bile acids specifically increase hepatitis c virus rna-replication胆汁酸专门增加丙型肝炎病毒rna复制
Bile Acids Specifically Increase Hepatitis C Virus RNA-
Replication
1 1 1 1 2
Patrick Chhatwal , Dorothea Bankwitz , Juliane Gentzsch , Anne Frentzen , Philipp Schult ,
2 1
Volker Lohmann , Thomas Pietschmann *
1 Department of Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Medical School Hannover and
the Helmholtz Centre for Infection Research, Hannover, Germany, 2 Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany
Abstract
Background: Hepatitis C virus (HCV) patients with high serum levels of bile acids (BAs) respond poorly to IFN therapy. BAs
have been shown to increase RNA-replication of genotype 1 but not genotype 2a replicons. Since BAs modulate lipid
metabolism including lipoprotein secretion and as HCV depends on lipids and lipoproteins during RNA-replication, virus
production and cell entry, BAs may affect multiple steps of the HCV life cycle. Therefore, we analyzed the influence of BAs on
individual steps of virus replication.
Methods: We measured replication of subgenomic genotype (GT) 1b and 2a RNAs as well as full-length GT2a genomes in
the presence of BAs using quantitative RT-PCR and luciferase assays. Cell entry was determined using HCV pseudoparticles
(HCVpp). Virus assembly and release were quantified using a core-specific ELISA. Replicon chimeras were employed to
characterize genotype-specific modulation of HCV by BAs. Lunet CD81/GFP-NLS-MAVS cells were used to determine
in
您可能关注的文档
- atrial tachyarrhythmia in rgs5-null mice心房在rgs5-null老鼠快速性心律失常.pdf
- atr-p53 restricts homologous recombination in response to replicative stress but does not limit dna interstrand crosslink repair in lung cancer cellsatr-p53限制同源重组,以应对dna复制的压力但不限制interstrand交联修复在肺癌细胞.pdf
- at-risk and recent-onset type 1 diabetic subjects have increased apoptosis in the cd4+cd25+high t-cell fraction危险和最近研究1型糖尿病患者增加了细胞凋亡的cd4 + cd25 + t细胞比例高.pdf
- atsofl1 and atsofl2 act redundantly as positive modulators of the endogenous content of specific cytokinins in arabidopsisatsofl1 atsofl2多余地采取积极的调节器的内生内容特定的细胞分裂素在拟南芥.pdf
- attack resilience of the evolving scientific collaboration network不断发展的科学合作网络的攻击能力.pdf
- attention increases the temporal precision of conscious perception verifying the neural-st2 model注意增加意识知觉的时间精度验证neural-st2模型.pdf
- attention deficit hyperactivity disorder in australian adults prevalence, persistence, conduct problems and disadvantage在澳大利亚成人注意缺陷多动障碍患病率、持久性,行为问题和缺点.pdf
- attentional performance, age and scholastic achievement in healthy children注意力的性能,在健康儿童年龄和学术成就.pdf
- attentional prioritization of infant faces is limited to own-race infants注意力优先级的婴儿脸仅限于自己种族婴儿.pdf
- attentive and pre-attentive processes in change detection and identification细心和pre-attentive过程改变探测和识别.pdf
- bili inhibits wntβ-catenin signaling by regulating the recruitment of axin to lrp6的招聘,并抑制wntβ-catenin信号通过调节轴蛋白lrp6.pdf
- bile salt-stimulated lipase plays an unexpected role in arthritis development in rodents胆汁salt-stimulated脂肪酶在啮齿动物关节炎发展起到了意想不到的作用.pdf
- bilingualism accentuates children's conversational understanding双语强调了儿童会话的理解.pdf
- bim links er stress and apoptosis in cells expressing mutant sod1 associated with amyotrophic lateral sclerosisbim链接er应激和凋亡细胞中表达sod1基因突变与肌萎缩性脊髓侧索硬化症有关.pdf
- bim mediates egfr tyrosine kinase inhibitor-induced apoptosis in lung cancers with oncogenic egfr mutationsbim调节表皮生长因子受体酪氨酸激酶inhibitor-induced细胞凋亡与致癌肺癌表皮生长因子受体突变.pdf
- bimodal and hysteretic expression in mammalian cells from a synthetic gene circuit双峰和滞后表达基因在哺乳动物细胞合成电路.pdf
- bim-mediated akt phosphorylation is a key modulator of arsenic trioxide-induced apoptosis in cisplatin-sensitive and -resistant ovarian cancer cells砷trioxide-induced bim-mediated一种蛋白激酶磷酸化是一个关键的调制器在防cisplatin-sensitive和卵巢癌细胞凋亡.pdf
- binary is typing for staphylococcus aureus二进制输入为金黄色葡萄球菌.pdf
- binding free energy landscape of domain-peptide interactions结合自由能景观domain-peptide交互.pdf
- binding of nir-conpk and nir-6t to astrocytomas and microglial cells evidence for a protein related to tspo绑定nir-conpk和nir-6t星形细胞瘤和小胶质细胞的证据tspo相关蛋白质.pdf
文档评论（0）