cardio-protection of salvianolic acid b through inhibition of apoptosis networkcardio-protection salvianolic酸通过抑制细胞凋亡网络b.pdfVIP

Cardio-Protection of Salvianolic Acid B through Inhibition of Apoptosis Network 1 1 1 1 1 1 1 2 Lingling Xu , Yanping Deng , Lixin Feng , Defang Li , Xiaoyan Chen , Chao Ma , Xuan Liu , Jun Yin , 1 1,2 1 1 1 1 Min Yang , Fukang Teng , Wanying Wu , Shuhong Guan , Baohong Jiang *, Dean Guo * 1 Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China, 2 Shenyang Pharmaceutical University, Shenyang, China Abstract Targeting cellular function as a system rather than on the level of the single target significantly increases therapeutic potency. In the present study, we detect the target pathway of salvianolic acid B (SalB) in vivo. Acute myocardial infarction (AMI) was induced in rats followed by the treatment with 10 mg/kg SalB. Hemodynamic detection and pathological stain, 2- dimensional electrophoresis, MALDI-TOF MS/MS, Western blot, pathway identification, apoptosis assay and transmission electron microscope were used to elucidate the effects and mechanism of SalB on cardioprotection. Higher SalB concentration was found in ischemic area compared to no-ischemic area of heart, correlating with improved heart function and histological structure. Thirty-three proteins regulated by SalB in AMI rats were identified by biochemical analysis and were classified as the components of metabolism and apoptosis networks. SalB protected cardiomyocytes from apoptosis, inhibited poly (ADP-ribose) polymerase-1 pathway, and improved the integrity of mitochondrial and nucleus of heart tissue during AMI. Furthermore, the prot