chemically-induced cancers do not originate from bone marrow-derived cells化学物质诱导癌症并不起源于骨骨髓来源的细胞.pdfVIP

Chemically-Induced Cancers Do Not Originate from Bone Marrow-Derived Cells 1. 2,3,4. 2 1 5 5 5 1 Hui Lin , Liang Hu , Leilei Chen , Hong Yu , Qi Wang , Ping Chen , Xiao-Tong Hu , Xiu-Jun Cai *, Xin-Yuan Guan2* 1 Department of General Surgery, Sir RunRun Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China, 2 Department of Clinical Oncology, State Key Laboratory for Liver Research, The University of Hong Kong, Hong Kong, Special Administrative Region, People’s Republic of China, 3 Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China, 4 National Engineering Research Center of Human Stem Cells, Changsha, China, 5 Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China Abstract Background: The identification and characterization of cancer stem cells (CSCs) is imperative to understanding the mechanism of cancer pathogenesis. Growing evidence suggests that CSCs play critical roles in the development and progression of cancer. However, controversy exists as to whether CSCs arise from bone marrow-derived cells (BMDCs). Methodology and Principal Findings: In the present study, n-nitrosodiethylamine (DEN) was used to induce tumor formation in female mice that received bone marrow from male mice. Tumor formation was induced in 20/26 mice, including 12 liver tumors, 6 lung tumors, 1 bladder tumor and 1 nasopharyngeal tumor. Through comparison of fluorescence in situ hybridization (FISH) results in corresponding areas from serial tumor sections