clinical validation of integrated nucleic acid and protein detection on an electrochemical biosensor array for urinary tract infection diagnosis临床验证集成核酸和蛋白质检测的电化学生物传感器阵列对尿路感染的诊断.pdfVIP

Clinical Validation of Integrated Nucleic Acid and Protein Detection on an Electrochemical Biosensor Array for Urinary Tract Infection Diagnosis 1,2 1,2 1 1,2 2 Ruchika Mohan , Kathleen E. Mach , Moran Bercovici , Ying Pan , Lakshmi Dhulipala , Pak Kin Wong3, Joseph C. Liao1,2* 1 Department of Urology and Bio-X Program, Stanford University School of Medicine, Stanford, California, United States of America, 2 Veterans Affairs Palo Alto Health Care System, Palo Alto, California, United States of America, 3 Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, Arizona, United States of America Abstract Background: Urinary tract infection (UTI) is a common infection that poses a substantial healthcare burden, yet its definitive diagnosis can be challenging. There is a need for a rapid, sensitive and reliable analytical method that could allow early detection of UTI and reduce unnecessary antibiotics. Pathogen identification along with quantitative detection of lactoferrin, a measure of pyuria, may provide useful information towards the overall diagnosis of UTI. Here, we report an integrated biosensor platform capable of simultaneous pathogen identification and detection of urinary biomarker that could aid the effectiveness of the treatment and clinical management. Methodology/Principal Findings: The integrated pathogen 16S rRNA and host lactoferrin detection using the biosensor array was performed on 113 clinical urine samples collected from patients at risk for complicated UTI. For pathogen detection, the biosensor used sandwich hybridization of capture and detector oligonucleotides to the target analyte, b