colorectal cancer migration and invasion initiated by microrna-106a结直肠癌迁移和入侵由微rna - 106 a.pdfVIP

Colorectal Cancer Migration and Invasion Initiated by microRNA-106a 1,2,3. 4. 5 1,2,3 1,2,3 1 Bo Feng , Tao Tao Dong , Lin Lin Wang , Hou Min Zhou , Hong Chao Zhao , Feng Dong *, Min Hua Zheng1,2,3* 1 Department of Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of China, 2 Shanghai Institute of Digestive Surgery, Shanghai, People’s Republic of China, 3 Shanghai Minimally Invasive Surgery Center, Shanghai, People’s Republic of China, 4 Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Ji’nan, Shandong, People’s Republic of China, 5 Shanghai Institute of Medical Genetics, Shanghai Children’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China Abstract MicroRNAs have been implicated in the regulation of several cellular signaling pathways of colorectal cancer (CRC) cells. Although emerging evidence proves that microRNA (miR)-106a is expressed highly in primary tumor and stool samples of CRC patients; whether or not miR-106a mediates cancer metastasis is unknown. We show here that miR-106a is highly expressed in metastatic CRC cells, and regulates cancer cell migration and invasion positively in vitro and in vivo. These phenotypes do not involve confounding influences on cancer cell proliferation. MiR-106a inhibits the expression of transforming growth factor-b receptor 2 (TGFBR2), leading to increased CRC cell migration and invasion. Importantly, miR- 106a expression levels in primary CRCs are correlated with clinical cancer progression. These observations indicate that miR- 1