comprehensive analysis of affymetrix exon arrays using bioconductor综合分析affymetrix使用bioconductor外显子数组.pdfVIP
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comprehensive analysis of affymetrix exon arrays using bioconductor综合分析affymetrix使用bioconductor外显子数组
Education
Comprehensive Analysis of Affymetrix Exon
Arrays Using BioConductor
*
Michał J. Okoniewski, Crispin J. Miller
million features: the previous generation of Gene-level arrays
had approximately 600,000. By removing the MM probes and
reducing the number of probes within a probeset from 11 to
4, the total probeset count has been increased to ;1.4
million, allowing probesets to be systematically placed along
the full length of each gene (see Box 2 for an overview of the
terminology; for more details on the design of the Affymetrix
platform see the ‘‘Learning Center’’ on Affymetrix’ Web site
[9] or one of the many review articles (e.g., [10])). The aim has
Introduction been to comprehensively target every known and predicted
exon in the human genome (Figure 1).
An important point to appreciate, particularly as feature
Why BioConductor? BioConductor [1] is a collection of
densities increase and arrays cover more
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